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Müller and macrophage-like cell interactions in an organotypic culture of porcine neuroretina.
| Tipo: | Artículo |
| Autores: | Fernandez-Bueno I, Pastor JC, Gayoso MJ, Alcalde I, Garcia MT. |
| Títuto Revista: | Molecular Vision |
| Referencia: | |
| Centro: | 03 - IOBA - UVA |
Müller and macrophage-like cell interactions in an organotypic culture of porcine neuroretina.
Source
University of Valladolid, Instituto Universitario de Oftalmobiologia Aplicada (IOBA), Campus Miguel Delibes s/n Valladolid, Valladolid, Spain. ifernandezb@ioba.med.uva.es
Abstract
PURPOSE:
To analyze the in vitro Müller cell modifications in an organotypic culture of porcine neuroretina in response to the addition of a blood-derived mononuclear fraction (MNF; monocytes and lymphocytes) as a source of macrophages.
METHODS:
Control and MNF-stimulated neuroretinal explants were examined at 3, 6, and 9 days of culture. Specimens were processed for epoxy-resin embedding and cryosectioning. Light and immunofluorescence microscopy were performed, using toluidine blue staining and antibodies against glial fibrillary acidic protein (GFAP), as a reactive gliosis marker, and cellular retinaldehyde-binding protein (CRALBP), as a Müller cell marker.
RESULTS:
Compared to controls, explants cocultured with MNF displayed increased cellular disorganization and larger tissue invasion of the subretinal space at 9 days of culture. Immunostaining of the MNF-treated explants revealed evidence of more reactive gliosis and greater number of GFAP-immunoreactive Müller cells that had increased width and processes extending into the subretinal space and forming a multilayer tissue. Astrocytes also responded to the MNF addition, producing extensions that invaded the neuroretinal outer layers.
CONCLUSIONS:
Addition of MNF stimulates modifications of Müller cells, producing a wider intraretinal reactive gliosis and tissue proliferation at the subretinal space (outer layers of the retina). These findings emphasize the role of macrophage-like cells in the production of changes in retinal structure observed after retinal detachment in humans.
- PMID: 19052655 [PubMed - indexed for MEDLINE]
- PMCID: PMC2593001
