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Müller and macrophage-like cell interactions in an organotypic culture of porcine neuroretina.

Tipo: Artículo
Autores: Fernandez-Bueno I, Pastor JC, Gayoso MJ, Alcalde I, Garcia MT.
Títuto Revista: Molecular Vision
Centro: 03 - IOBA - UVA
Mol Vis. 2008;14:2148-56. Epub 2008 Nov 28.

Müller and macrophage-like cell interactions in an organotypic culture of porcine neuroretina.


University of Valladolid, Instituto Universitario de Oftalmobiologia Aplicada (IOBA), Campus Miguel Delibes s/n Valladolid, Valladolid, Spain. ifernandezb@ioba.med.uva.es



To analyze the in vitro Müller cell modifications in an organotypic culture of porcine neuroretina in response to the addition of a blood-derived mononuclear fraction (MNF; monocytes and lymphocytes) as a source of macrophages.


Control and MNF-stimulated neuroretinal explants were examined at 3, 6, and 9 days of culture. Specimens were processed for epoxy-resin embedding and cryosectioning. Light and immunofluorescence microscopy were performed, using toluidine blue staining and antibodies against glial fibrillary acidic protein (GFAP), as a reactive gliosis marker, and cellular retinaldehyde-binding protein (CRALBP), as a Müller cell marker.


Compared to controls, explants cocultured with MNF displayed increased cellular disorganization and larger tissue invasion of the subretinal space at 9 days of culture. Immunostaining of the MNF-treated explants revealed evidence of more reactive gliosis and greater number of GFAP-immunoreactive Müller cells that had increased width and processes extending into the subretinal space and forming a multilayer tissue. Astrocytes also responded to the MNF addition, producing extensions that invaded the neuroretinal outer layers.


Addition of MNF stimulates modifications of Müller cells, producing a wider intraretinal reactive gliosis and tissue proliferation at the subretinal space (outer layers of the retina). These findings emphasize the role of macrophage-like cells in the production of changes in retinal structure observed after retinal detachment in humans.

PMID: 19052655 [PubMed - indexed for MEDLINE]

PMCID: PMC2593001